LABORATORY TECHNOLOGY

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HIELSCHER: ultrasonic laboratory device for homogenizing, dispersing, cell lysis...

Ultrasonic processors for laboratories are mostly used, when preparing samples for analysis. In addition to that, the development of new techniques on the basis of the cavitation effect is getting more and more important.  

Applications in the laboratory  

Working principle

The selection of the ultrasonic processor and sonotrodes mainly complies with the volume of the samples to be sonicated. The offered power range for the laboratory includes devices of 50, 100, 200 and 400 watts and is suitable for small droplets but also for several liters. For bigger volumes we offer industrial processors with a power range of up to 16kW per unit.

 
UP50H, from 0.01 to 250 ml, 50W UP100H, from 0.01 to 500 ml, 100W

    Donwload more informations and prices for UP50H here...

    Donwload more informations and prices for UP50H here...

 
  UP200Ht : Version generator and transducer combined

 UP200St-D and UP200St-T: Version generator and transducer separated

 for sample volume between 2 ml and 1000 ml, 
power 200W

!!!  new  !!!
New model 200Watts easy to use, economical and with functions innovations
Version generator and transducer combined

UP200Ht_S26d14_HIELSCHER_IMLAB.jpg (43996 bytes)  UP200Ht_SPB-L_HIELSCHER_IMLAB.jpg (71712 bytes)
Models UP200Ht , from 2 ml to 1000 ml,  200 watts


    Donwload more informations and prices for UP200Ht here...

    Download the specifications for UP200Ht here...

 

!!!  new  !!!
New model 200Watts easy to use, economical and with functions innovations
Version generator and transducer separated

Models UP200St-G and UP200St-T, from 2 ml to 1000 ml,  200 watts


    Donwload more informations and prices for UP200St-S and UP20St-T here...

    Download the specifications for UP200St-G and UP200St-T here...

 
UP200H and UP200S, from 0.1 to 1000 ml, 200W UP400S, from 5 to 2000 ml, 400W
 

    Donwload more informations and prices for UP200S here...

    Donwload more informations and prices for UP400S here...

 
HIELSCHER
Applications in the laboratory               

In biology, molecular biology, biochemistry, biotech­nology and in the medical range ultrasound is used for the disruption of cells for the isolation of DNA, RNA or proteins and for the homogenizing of samples. Muscle tissue, liver tissue or lymphocytes as well as vegetable cells can be disrupted for analysis purposes or for the use of their contents.

The exact control of the amplitude allows for an isolation of the cell nuclei, too. Sensitive materials can be homogenized or disrupted gently.

The sonotrodes can be treated in autoclaves and can be cleaned easily due to their simple geometry, so that contamination does not take place. For special application cases we offer devices and accessories for indirect sonication, so that the sample stays isolated.

The separation of viruses from bacteria or the extraction of active agents from vegetable cells are exemplary for the practice in the pharmaceutical industry.

In the food industry cheese, meat and sausage samples, fruits, or vegetables are homogenized in order to test the quality for consumer protection. In this branch of industry ultrasound is also used for the production of oil/water emulsions, in which very fine droplet sizes of under 200nm can be achieved. The degassing of beverages is useful for analysis. The reducing of germs in liquids is already possible in low temperatures by the use of ultrasound.

Environmental standard tests are the homogenizing of water-, waste water- and soil samples in order to detect or determine oils, radio-active parts or the content of fertilizers.

In the chemical industry ultrasound is used for the production of suspensions, emulsions, for the interference of crystallization or polymerization and for the acceleration of chemical reactions or dissolving processes.

deagglomerating and sieving by means of ultrasound are applications, that are in particular helpful, when manufacturing coatings.

A better permeation of pigments and an even distribution can be reached with ultrasonic applications such as wet coloring and impregnating.

Cleaning is the most common ultrasonic application. The following described laboratory devices can also be set in use for special cleaning tasks, that demand a high ultrasonic intensity on small surfaces e.g. bores can be cleaned by ultrasound, when using thin-bodied sonotrodes.
Working principle               

The operation of our ultrasonic processors is most simple. The amplitude and therewith the power can be adjusted from 20 to 100%. With an adjustable pulse from 0 up to 1 the operation can be stopped in defined regular intervals, so that problems as regards the heating-up and foaming can be avoided. The automatic determination of the ideal frequency makes a manual precision adjustment unnecessary. Independent of the sonotrode’s load, the adjusted amplitude remains constant. That creates reproducible conditions and also high robustness as the devices are then steady-state proof even when operated in air.

Sonotrodes                        

The sonotrodes are the tools that transmit the ultrasound into the liquid. The selection of the sonotrodes depends on the sample volume and on the required ultrasound intensity. Thin-bodied sonotrodes achieve very high amplitudes up to 250µm, that result in very high ultrasound power densities under the oscillating end surface. A small but very intensive cavitation zone is generated. The bigger the diameter, the higher is the ultrasound power, that is transmitted via the consequently bigger end face of the sonotrode. The ultrasound power density i.e. the ratio between power and oscillating surface becomes smaller. The cavitation zone becomes larger but less intensive.

A titanium alloy has proven to be the best sonotrode material as it allows very high amplitudes and as it is resistant to most liquids. Therefore this titanium alloy is the standard material for our sonotrodes and the ultrasonic processors are adjusted to it. For special application cases we manufacture sonotrodes made of stainless steel, glass or ceramics. The max. amplitudes of the sonotrodes made of these materials are significantly below those of the titanium sonotrodes.

The lengths of the sonotrodes are determined by lambda/half. If the ultrasonic processor has the frequency of 24kHz, the titanium sonotrode has approx. the length 100mm but can also be manufactured in manifold lengths. Sonotrodes with O-rings or oscillating-free flanges are used for the input of ultrasound in flow cells or in closed or pressurized systems.

The cavitation, that takes place at the sonotrode surface, results in an abrasional effect. At the max. amplitude, approx. 1mm of the sonotrode abrades within 1000 hours of operation in water.The frequency scanning system of our ultrasonic processors raises then automatically the frequency, so that an abrasion of up to 5mm of the sonotrode does not result in a considerable loss of power.

 

Selection of the combination sonificator-sonotrode in function of the sample volume ( ml ) to be treated                             

 

UP50H

UP100H

UP200H

UP200S

UP400S

Sample volume 
( ml )

Diamètre de la sonotrode ( mm )

0.01-0.50

0.5

0.5

 

 

 

0.1-5.0

1

1

1

1

 

2-50

2

2

2

2

 

5-100

3

3

3

3

3

10-250

7

7

7

7

7

20-500

 

10

 

 

 

50-1000

 

 

14

14

14

10-2000

 

 

 

 

22

Download the complete documentation HIELSCHER here  in pdf

 

IMLAB bvba
Oude Vijvers 1
B - 3370 Boutersem
Belgique
info@imlab.be
www.imlab.be

Tél.:  016 - 73 55 72
Fax : 016 - 73 55 87

IMLAB sarl
Centre d'Affaires de l'Horlogerie
48 rue des Canonniers
F - 59000 Lille
France
imlab@wanadoo.fr
www.imlab.com

Tél.:  03 20 55 19 11
Fax : 03 20 55 20 85